Details

Autor(en) / Beteiligte

• Honma, Youhei
• Yamakawa, Takashi

Titel

High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter

Ist Teil von

• Plant cell reports, 2019-11, Vol.38 (11), p.1417-1426

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Key message We developed transgenic sweet potato with Spo min (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spo min were higher than those by CaMV 35S promoter. We developed transgenic sweet potato ( Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spo min (sucrose-inducible minimal promoter)-GUS gene-fused constructs with signal peptides for sorting to cytosol, apoplast and ER, and we analyzed the GUS expression pattern of cut tissue after sucrose treatment. Induced GUS activities by Spo min were several hundred times higher than those by the CaMV 35S promoter. Also, GUS activities in storage roots induced with a Spo min –cytosol-GUS construct were higher than those with either Spo min –apoplast or –ER-GUS constructs. The induced GUS activities by Spo min were higher in storage roots without sucrose treatment than those with sucrose treatment. Chilling (4 °C) storage roots with Spo min constructs for 4 weeks produced higher GUS activities than in storage roots stored at 25 °C for 4 weeks. The calculated maximum GUS content in the storage roots was up to about 224.2 μg/g fresh weight. The chilling treatment increased the free sucrose content in the storage roots, and this increase in endogenous sugar levels induced increased GUS activities in the storage roots. Therefore, Spo min appears to be a useful promoter to develop protein production systems using sweet potato variety Kokei no. 14 storage roots by postharvest treatment.