Autor(en)
He, Zhen; Gharaibeh, Raad Z; Newsome, Rachel C; Pope, Jllian L; Dougherty, Michael W; Tomkovich, Sarah; Pons, Benoit; Mirey, Gladys; Vignard, Julien; Hendrixson, David R; Jobin, Christian
Titel
Campylobacter jejuni promotes colorectal tumorigenesis through the action of cytolethal distending toxin
Teil von
  • Gut, 2019-02-01, Vol.68 (2), p.289-300
Ort / Verlag
LONDON: BMJ PUBLISHING GROUP
Links zum Volltext
Quelle
Web of Science
Beschreibungen
Objective Campylobacter jejuni produces a genotoxin, cytolethal distending toxin (CDT), which has DNAse activity and causes DNA double-strand breaks. Although C. jejuni infection has been shown to promote intestinal inflammation, the impact of this bacterium on carcinogenesis has never been examined. Design Germ-free (GF) Apc(Min/+) mice, fed with 1% dextran sulfate sodium, were used to test tumorigenesis potential of CDT-producing C. jejuni. Cells and enteroids were exposed to bacterial lysates to determine DNA damage capacity via gamma H2AX immunofluorescence, comet assay and cell cycle assay. To examine the interplay of CDT-producing C. jejuni, gut microbiome and host in tumorigenesis, colonic RNA-sequencing and faecal 16S rDNA sequencing were performed. Rapamycin was administrated to investigate the prevention of CDT-producing C. jejuni-induced tumorigenesis. Results GF Apc(Min/+) mice colonised with human clinical isolate C. jejuni81-176 developed significantly more and larger tumours when compared with uninfected mice. C. jejuni with a mutated cdtB subunit, mutcdtB, attenuated C. jejuni-induced tumorigenesis in vivo and decreased DNA damage response in cells and enteroids. C. jejuni infection induced expression of hundreds of colonic genes, with 22 genes dependent on the presence of cdtB. The C. jejuni-infected group had a significantly different microbial gene expression profile compared with the mutcdtB group as shown by metatranscriptomic data, and different microbial communities as measured by 16S rDNA sequencing. Finally, rapamycin could diminish the tumorigenic capability of C. jejuni. Conclusion Human clinical isolate C. jejuni 81-176 promotes colorectal cancer and induces changes in microbial composition and transcriptomic responses, a process dependent on CDT production.
Format
Sprache(n)
Englisch
Identifikator(en)
ISSN: 0017-5749
ISSN: 1468-3288
DOI: 10.1136/gutjnl-2018-317200
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Schlagwörter
Gastroenterology & Hepatology, Life Sciences & Biomedicine, Science & Technology
Systemstelle
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