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Autor(en) / Beteiligte
Titel
Efficacy of Montanide™ ISA 763 A VG as aquatic adjuvant administrated with an inactivated Vibrio harveyi vaccine in turbot (Scophthalmus maximus L.)
Ist Teil von
  • Fish & shellfish immunology, 2019-01, Vol.84, p.56-61
Ort / Verlag
England: Elsevier Ltd
Erscheinungsjahr
2019
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
  • Turbot (Scophthalmus maximus L.) is a commercially important fish species in China. Despite of its great economic potential, fish farms often suffer severe economic losses due to certain fish diseases. Vaccination has become a common strategy to prevent diseases caused by pathogens in aquaculture industry. However, no inactivated vaccine against Vibrio harveyi of turbot has been reported so far. In this study, we developed an inactivated vaccine using formalin-killed cells of V. harveyi and the efficacy of a commercial adjuvant Montanide™ ISA 763 A VG on the inactivated vaccine was evaluated. We found that with an optimum vaccine dosage at 1.0 × 108 CFU/fish, a high relative percent survival (RPS) more than 75% was observed at 4 weeks post vaccination (w.p.v.). Moreover, enhanced antibody titer, lysozyme activity, total serum protein and antibacterial property in sera of vaccinated fish were observed at 4, 8, 12 and 16 w.p.v. In conclusion, we developed an efficient inactivated vaccine against V. harveyi in turbot, which not only induced humoral immunity, but also enhanced initial innate immune response for long-term protection. •An inactived Vibrio harveyi vaccine is developed in turbot.•The inactivated vaccine can induce efficient protection lasting for a long term.•The inactivated vaccine can induce humoral immunity and strong innate immune responses.
Sprache
Englisch
Identifikatoren
ISSN: 1050-4648, 1095-9947
eISSN: 1095-9947
DOI: 10.1016/j.fsi.2018.09.024
Titel-ID: cdi_pubmed_primary_30201447

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