Details

Autor(en) / Beteiligte

• Kuang, Dai
• Zhang, Jianmin
• Xu, Xuebin
• Shi, Weimin
• Chen, Sheng
• Yang, Xiaowei
• Su, Xudong
• Shi, Xianming
• Meng, Jianghong

Titel

Emerging high-level ciprofloxacin resistance and molecular basis of resistance in Salmonella enterica from humans, food and animals

Ist Teil von

• International journal of food microbiology, 2018-09, Vol.280, p.1-9

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Disease caused by nontyphoidal serotypes of Salmonella enterica is the leading cause of foodborne illness worldwide. Many serotypes have also developed resistance to antimicrobials commonly used for the treatment of salmonellosis. Here we analyze 179 ciprofloxacin-resistant isolates identified among 3680 Salmonella isolated from humans, food, animals, and water collected in Shanghai, China. They were assessed for antimicrobial susceptibility, plasmid-mediated quinolone resistance (PMQR) determinants, and mutations in quinolone resistance determination regions (QRDRs); genetic relatedness was examined using PFGE. Ciprofloxacin resistance in Salmonella increased from 2.3% in 2006 to 5.9% in 2012. Multidrug resistance was common, and most carried mutations in QRDR (97.2%) and PMQR determinants (71.5%). Mutations frequently included changes in gyrA: Ser83Phe (53.6%) and Asp87Asn (35.8%), and in parC: Thr57Ser (53.1%) and Ser80Arg (44.1%). Mutations in parC and parE without changes in gyrA were identified in S. Derby and most S. Thompson. Among PMQR determinants, aac(6′)-Ib-cr (62.0%) and oqxA/oqxB (33.5%/33.0%) were most common, and conferred resistance without target mutations in five S. Typhimurium isolates. PFGE analysis revealed that S. Typhimurium, isolated from pork and aquatic products, and S. Indiana, isolated from chicken, were highly similar to isolates from humans, suggesting these products be the major source of ciprofloxacin-resistant infections. Our findings highlight the important role QRDRs and PMQR play in ciprofloxacin resistance of Salmonella, and reveal the potential sources of the pathogen associated with human infections. •Significant increase of ciprofloxacin-resistant Salmonella enterica in China.•Mechanisms of ciprofloxacin resistance vary among different serovars of Salmonella.•Plasmid-mediated quinolone resistance determinants alone can confer cipro resistance.•Pork, aquatic and chicken products were the major source of human salmonellosis.