Details

Autor(en) / Beteiligte

• Wang, Ji
• Wu, Tong
• Fang, Xiaobin
• Min, Weihong
• Yang, Zhennai

Titel

Characterization and immunomodulatory activity of an exopolysaccharide produced by Lactobacillus plantarum JLK0142 isolated from fermented dairy tofu

Ist Teil von

• International journal of biological macromolecules, 2018-08, Vol.115, p.985-993

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• A purified neutral exopolysaccharide (EPS) designated as EPS0142 was obtained from Lactobacillus plantarum JLK0142. EPS0142 consisted of glucose and galactose in an approximate molar ratio of 2.13:1.06 and had a molecular weight of 1.34 × 105 Da. The FT-IR spectrum showed that EPS0142 had a typical polysaccharide absorption pattern. 1H NMR and 13C NMR spectra analysis showed the presence of N-acetylated sugar residues. EPS0142 had no toxic effects on RAW 264.7 cells and significantly improved their phagocytic activity and NO secretion in vitro. Further in vivo studies revealed that the spleen index and splenic lymphocyte proliferation activities of the cyclophosphamide-induced immunosuppression mice treated with a middle-dose (50 mg/kg body weight) or a high-dose (100 mg/kg body weight) of EPS0142 were significantly increased (P < 0.01). In addition, the intestinal immunoglobulin A (sIgA) content and the serum levels of the cytokines, IL-2 and TNF-α, were also significantly (P < 0.05) improved in the high-dose EPS0142 group compared to that in the model control group. These data indicate that the EPS isolated from L. plantarum JLK0142 can effectively improve the immunomodulatory activity of RAW 264.7 cells and stimulate the immune system in cyclophosphamide-induced immunosuppressed mice. •A neutral EPS0142 from Lactobacillus plantarum EPS0142 was composed of glucose and galactose.•Functional groups were identified using FT-IR and NMR (1H &13C).•EPS0142 significantly improved phagocytic activity of macrophages and the secretion of NO.•EPS0142 increased immunomodulatory activity in cyclophosphamide-induced immunosuppressed mice.