Details

Autor(en) / Beteiligte

• Zhou, Haisheng
• Zhang, Meihong
• Tian, Xue
• Shao, Hongxia
• Qian, Kun
• Ye, Jianqiang
• Qin, Aijian

Titel

Identification of a novel recombinant virulent avian infectious bronchitis virus

Ist Teil von

• Veterinary microbiology, 2017-02, Vol.199, p.120-127

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

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Beschreibungen/Notizen

• •A novel IBV variant CK/CH/2010/JT-1 was identified.•A novel genotypic cluster IBV has emerged in China.•Isolate CK/CH/2010/JT-1 originated from homologous RNA recombination.•Isolate CK/CH/2010/JT-1 is highly virulent. The emergence of new infectious bronchitis virus (IBV) variants is often disastrous in the poultry industry. In this study, an IBV, CK/CH/2010/JT-1, was isolated from an H120- and 4/91-IBV-vaccinated flock in China. Antisera against vaccine strains H120 and 4/91 could not provide effective protection against CK/CH/2010/JT-1 in virus neutralization assays. CK/CH/2010/JT-1 could cause 43.75% mortality with respiratory and severe renal lesions in inoculated chickens. Phylogenetic analysis of the S1 gene showed that CK/CH/2010/JT-1 and 31 other isolates could be grouped as a new genotypic cluster. Recombination analysis revealed that three recombination events could be found in the genome of CK/CH/2010/JT-1 at positions 24709-365, 17160-19811 and 21136-21770. Whole-genome sequence analysis showed that CK/CH/2010/JT-1 originated from multiple template switches among QX-like, CK/CH/LSC/99I-, tl/CH/LDT3/03- and 4/91-type IBVs. All of these data demonstrated that CK/CH/2010/JT-1 is a new recombinant genotype IBV with high virulence. Our findings suggest that the surveillance of new genotype strains of IBV is very important for developing more effective anti-IBV strategies.