Details

Autor(en) / Beteiligte

• Hemme, Ryan R.
• Poole-Smith, B. Katherine
• Hunsperger, Elizabeth A.
• Felix, Gilberto E.
• Horiuchi, Kalanthe
• Biggerstaff, Brad J.
• Lopez-Ortiz, Ricardo
• Barrera, Roberto

Titel

Non-human primate antibody response to mosquito salivary proteins: Implications for dengue virus transmission in Puerto Rico

Ist Teil von

• Acta tropica, 2016-12, Vol.164, p.369-374

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •Twenty-two dengue virus infected non-human primates were evaluated for exposure to mosquito bites.•Specific monkey antibody response to Aedes aegypti, Aedes mediovittatus, Aedes taeniorhynchus, and Culex quinquefasciatus salivary gland extract was evaluated.•More non-human primates were positive for exposure to Aedes mediovittatus than any other mosquito species.•Non-human primates and mosquitoes may be able to sustain dengue virus transmission during inter-epidemic periods.•Mammalian antibody response to mosquito salivary glands has potential to incriminate cryptic or secondary vectors of disease causing pathogens. An important step to incriminate a mosquito as a vector of a disease pathogen is finding evidence of direct contact between the mosquito and humans. Typically, this is accomplished through landing/biting catches, or host blood meal analysis in engorged mosquitoes via immunologic assays. An alternate approach is to identify the presence of specific mosquito anti-saliva protein antibodies in the blood of exposed hosts. Following the discovery of dengue infected, free roaming non-human primates in Puerto Rico, we investigated which mosquito species had bitten these primates using a serologic assay. Serum samples from 20 patas monkeys (Erythrocebus patas) and two rhesus macaques (Macaca mulatta) were used to evaluate mosquito bite exposure to Aedes aegypti, Aedes mediovittatus, Aedes taeniorhynchus, and Culex quinquefasciatus mosquitoes. Of 22 non-human primates examined 20 (90%), 17 (77%), 13 (59%), and 7 (31%) were positive for exposure to Ae. mediovittatus, Cx. quinquefasciatus, Ae. taeniorhynchus, and Ae. aegypti, respectively. Our findings indicated that free-roaming primates in Puerto Rico were exposed to the bites of one proven dengue vector, Ae. aegypti and one potential dengue vector, Ae. mediovittatus.