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Details

Autor(en) / Beteiligte
Titel
Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients
Ist Teil von
  • Tuberculosis (Edinburgh, Scotland), 2016-09, Vol.100, p.89-94
Ort / Verlag
Scotland: Elsevier Ltd
Erscheinungsjahr
2016
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
  • Summary Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis ( M.tb ) during infection and treatment. However, whether M.tb transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M.tb transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2,179 M.tb transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value <0.05, but none were significant after correction for multiple testing. Categorical enrichment analysis indicated that expression of the hypoxia-responsive DosR regulon was higher in BAL than in sputum. M.tb transcription in BAL and sputum was distinct from both aerobic growth and NRP-2, with a range of 396-1020 transcripts significantly differentially expressed after multiple testing correction. Collectively, our results indicate that M.tb transcription in sputum approximates M.tb transcription in the lung. Minor differences between M.tb transcription in BAL and sputum suggested lower oxygen concentrations or higher nitric oxide concentrations in BAL. M.tb- targeted transcriptional profiling of sputa may be a powerful tool for understanding M.tb pathogenesis and monitoring treatment responses in vivo.

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