Details

Autor(en) / Beteiligte

• Stadler, Philipp
• Blöschl, Günter
• Vogl, Wolfgang
• Koschelnik, Juri
• Epp, Markus
• Lackner, Maximilian
• Oismüller, Markus
• Kumpan, Monika
• Nemeth, Lukas
• Strauss, Peter
• Sommer, Regina
• Ryzinska-Paier, Gabriela
• Farnleitner, Andreas H.
• Zessner, Matthias

Titel

Real-time monitoring of beta-d-glucuronidase activity in sediment laden streams: A comparison of prototypes

Ist Teil von

• Water research (Oxford), 2016-09, Vol.101, p.252-261

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Detection of enzymatic activities has been proposed as a rapid surrogate for the culture-based microbiological pollution monitoring of water resources. This paper presents the results of tests on four fully automated prototype instruments for the on-site monitoring of beta-d-glucuronidase (GLUC) activity. The tests were performed on sediment-laden stream water in the Hydrological Open Air Laboratory (HOAL) during the period of March 2014 to March 2015. The dominant source of faecal pollution in the stream was swine manure applied to the fields within the catchment. The experiments indicated that instrument pairs with the same construction design yielded highly consistent results (R2 = 0.96 and R2 = 0.94), whereas the results between different designs were less consistent (R2 = 0.71). Correlations between the GLUC activity measured on-site and culture-based Escherichia coli analyses over the entire study period yielded R2 = 0.52 and R2 = 0.47 for the two designs, respectively. The correlations tended to be higher at the event scale. The GLUC activity was less correlated with suspended sediment concentrations than with E. coli, which is interpreted in terms of indicator applicability and the time since manure application. The study shows that this rapid assay can yield consistent results over a long period of on-site operation in technically challenging habitats. Although the use of GLUC activity as a proxy for culture-based assays could not be proven for the observed habitat, the study results suggest that this biochemical indicator has high potential for implementation in early warning systems. [Display omitted] •On-site GLUC measurements can be continuously conducted in sediment-laden water.•Different prototype-constructions yield comparable measurement results.•GLUC signals indicate potential fecal pollution of stream water in near-real time.•On-site GLUC measurements are not a proxy for culture-based Escherichia coli analyses.