Details

Autor(en) / Beteiligte

• Ladd-Acosta, Christine
• Shu, Chang
• Lee, Brian K.
• Gidaya, Nicole
• Singer, Alison
• Schieve, Laura A.
• Schendel, Diana E.
• Jones, Nicole
• Daniels, Julie L.
• Windham, Gayle C.
• Newschaffer, Craig J.
• Croen, Lisa A.
• Feinberg, Andrew P.
• Daniele Fallin, M.

Titel

Presence of an epigenetic signature of prenatal cigarette smoke exposure in childhood

Ist Teil von

• Environmental research, 2016-01, Vol.144 (Pt A), p.139-148

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Prenatal exposure to tobacco smoke has lifelong health consequences. Epigenetic signatures such as differences in DNA methylation (DNAm) may be a biomarker of exposure and, further, might have functional significance for how in utero tobacco exposure may influence disease risk. Differences in infant DNAm associated with maternal smoking during pregnancy have been identified. Here we assessed whether these infant DNAm patterns are detectible in early childhood, whether they are specific to smoking, and whether childhood DNAm can classify prenatal smoke exposure status. Using the Infinium 450K array, we measured methylation at 26 CpG loci that were previously associated with prenatal smoking in infant cord blood from 572 children, aged 3–5, with differing prenatal exposure to cigarette smoke in the Study to Explore Early Development (SEED). Striking concordance was found between the pattern of prenatal smoking associated DNAm among preschool aged children in SEED and those observed at birth in other studies. These DNAm changes appear to be tobacco-specific. Support vector machine classification models and 10-fold cross-validation were applied to show classification accuracy for childhood DNAm at these 26 sites as a biomarker of prenatal smoking exposure. Classification models showed prenatal exposure to smoking can be assigned with 81% accuracy using childhood DNAm patterns at these 26 loci. These findings support the potential for blood-derived DNAm measurements to serve as biomarkers for prenatal exposure. •An epigenetic signature of prenatal exposure to cigarette smoke, originally discovered in other studies of cord blood at birth, is detectable in childhood blood samples at ages 3–5.•Prenatal smoking-associated loci do not show similar patterns or magnitudes of DNA methylation differences for other prenatal exposures.•DNA methylation patterns from 26 CpG sites at 3–5 years of age can accurately classify prenatal exposure to smoking.