Details

Autor(en) / Beteiligte

• Jaafar, Rzgar M.
• Chettri, Jiwan K.
• Dalsgaard, Inger
• Al-Jubury, Azmi
• Kania, Per W.
• Skov, Jakob
• Buchmann, Kurt

Titel

Effects of adjuvant Montanide™ ISA 763 A VG in rainbow trout injection vaccinated against Yersinia ruckeri

Ist Teil von

• Fish & shellfish immunology, 2015-12, Vol.47 (2), p.797-806

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Enteric redmouth disease (ERM) caused by the fish pathogen Yersinia ruckeri is a major threat to freshwater production of rainbow trout (Oncorhynchus mykiss) throughout all life stages. Injection vaccination of rainbow trout against Y. ruckeri infection has been shown to confer better protection compared to the traditionally applied immersion vaccination. It may be hypothesized, based on experience from other vaccines, that adjuvants may increase the protective level of ERM injection vaccines even more. Controlled comparative vaccination studies have been performed to investigate effects of the oil adjuvant Montanide™ ISA 763 A VG (Seppic) when added to an experimental Y. ruckeri bacterin (containing both biotype 1 and 2 of serotype O1). A total of 1000 fish with mean weight 19 g was divided into five different groups (in duplicated tanks 2 × 100 fish per group) 1) non-vaccinated control fish (NonVac), 2) fish injected with a commercial vaccine (AquaVac® Relera™) (ComVac), 3) fish injected with an experimental vaccine (ExpVac), 4) fish injected with an experimental vaccine + adjuvant (ExpVacAdj) and 5) fish injected with adjuvant alone (Adj). Injection of the experimental vaccine (both adjuvanted and non-adjuvanted) induced a significantly higher antibody (IgM) level, increased occurrence of IgM+ cells in spleen tissue and significant up-regulation of several immune genes. Additional experiments using a higher challenge dosage suggested an immune enhancing effect of the adjuvant as the challenge produced 100% mortality in the NonVac group, 60% mortality in both of ComVac and Adj groups and only 13 and 2.5% mortalities in the ExpVac and the ExpVacAdj groups, respectively. •Protection of aquacultured rainbow trout against yersiniosis will be better achieved through injection vaccination.•Adjuvanted vaccines with Montanide™ ISA 763 A VG revealed excellent protection following challenge with Yersinia ruckeri.•A clear cooperation between the humoral and cellular element of innate and adaptive immunity was evident in vaccinated fish.•No clear shift of the immune response towards a Th1, Th2, Th17 or Treg type was seen.•Rainbow trout clearly possess specific immunity, as characterized by antigen specificity and immunological memory.