Details

Autor(en) / Beteiligte

• Balti, Rafik
• Bougatef, Ali
• Sila, Assaâd
• Guillochon, Didier
• Dhulster, Pascal
• Nedjar-Arroume, Naima

Titel

Nine novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) muscle protein hydrolysates and antihypertensive effect of the potent active peptide in spontaneously hypertensive rats

Ist Teil von

• Food chemistry, 2015-03, Vol.170, p.519-525

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Purification of nine novel ACE inhibitory peptides from cuttlefish muscle protein.•Molecular masses and sequences were determined using ESI–MS and ESI–MS/MS.•The most potent peptide showed no cytotoxicity and was evaluated as a non-competitive inhibitor.•The most potent peptide showed a clear decrease in SBP and DBP in SHR.•These peptides are a potential benefit in the prevention of hypertension. This study aimed to identify novel ACE inhibitory peptides from the muscle of cuttlefish. Proteins were hydrolyzed and the hydrolysates were then subjected to various types of chromatography to isolate the active peptides. Nine ACE inhibitory peptides were isolated and their molecular masses and amino acid sequences were determined using ESI–MS and ESI–MS/MS, respectively. The structures of the most potent peptides were identified as Val-Glu-Leu-Tyr-Pro, Ala-Phe-Val-Gly-Tyr-Val-Leu-Pro and Glu-Lys-Ser-Tyr-Glu-Leu-Pro. The first peptide displayed the highest ACE inhibitory activity with an IC50 of 5.22μM. Lineweaver–Burk plots suggest that Val-Glu-Leu-Tyr-Pro acts as a non-competitive inhibitor against ACE. Furthermore, antihypertensive effects in spontaneously hypertensive rats (SHR) also revealed that oral administration of Val-Glu-Leu-Tyr-Pro can decrease systolic blood pressure significantly (p<0.01). These results suggest that the Val-Glu-Leu-Tyr-Pro would be a beneficial ingredient for nutraceuticals and pharmaceuticals acting against hypertension and its related diseases.