UB Paderborn / Katalog / Details

Autor(en) / Beteiligte

Titel

Combination of MIDGE-Th1 DNA vaccines with the cationic lipid SAINT-18: Studies on formulation, biodistribution and vector clearance

Ist Teil von

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

Highlights • MIDGE-Th1/SAINT-18 lipoplexes were characterized in vitro and in vivo. • Transfection efficiency of lipoplexes increased with SAINT-18 amount in vitro. • Lipoplexes containing lower amount of SAINT-18 were most immunogenic in mice. • Formulation with SAINT-18 resulted in better DNA uptake into draining lymph nodes. • MIDGE-Th1/SAINT-18 lipoplexes were well tolerated in mice and rats.

Sprache: Englisch
Identifikatoren: ISSN: 0264-410X
eISSN: 1873-2518
DOI: 10.1016/j.vaccine.2014.03.048
Titel-ID: cdi_pubmed_primary_24681271

Format: –
Schlagworte: Allergy and Immunology, Animals, Antibodies, Viral - blood, Applied microbiology, Biodistribution, Biological and medical sciences, Cationic lipid, Cations, Cell culture, Deoxyribonucleic acid, DNA, DNA vaccine, Efficiency, Female, Fundamental and applied biological sciences. Psychology, Gene expression, Genetic Vectors - chemistry, Genetic Vectors - pharmacokinetics, Hepatitis B Surface Antigens - immunology, Immunoglobulin G - blood, Lipids, Lymph Nodes - immunology, Male, Mice, Mice, Inbred BALB C, Microbiology, MIDGE-Th1, Particle size, Plasmids, Pyridinium Compounds - chemistry, Pyridinium Compounds - pharmacokinetics, Ratios, Rats, Rats, Wistar, SAINT-18, Th1 Cells - immunology, Tissue Distribution, Transfection, Transfection reagent, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects), Vaccines, DNA - immunology, Vaccines, DNA - pharmacokinetics

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