Details

Autor(en) / Beteiligte

• Kim, Kyung-Tae
• Rioux, Laurie-Eve
• Turgeon, Sylvie L.

Titel

Alpha-amylase and alpha-glucosidase inhibition is differentially modulated by fucoidan obtained from Fucus vesiculosus and Ascophyllum nodosum

Ist Teil von

• Phytochemistry (Oxford), 2014-02, Vol.98, p.27-33

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Starch digesting enzyme inhibition was induced by fucoidan extracted from brown seaweeds: α-amylase and α-glucosidase inhibition induced by fucoidan at 5 and 0.05mg/mL, respectively. •Fucoidan is an efficient inhibitor of α-amylase and α-glucosidase.•The inhibitory activity varied among harvesting period and the targeted enzyme.•Fucoidan from A. nodosum inhibited both enzymes.•Fucoidans from F. vesiculosus inhibited only α-glucosidase.•Each species has its own optimal period of harvesting for enzymatic inhibition. Fucoidan is a water-soluble, negatively charged, biologically active polysaccharide found in great abundance in brown marine algae. However, the inhibition of α-amylase and α-glucosidase by fucoidan derived from two algal species (Ascophyllum nodosum and Fucus vesiculosus) harvested at different periods (accounting for seasonal and yearly variations) has never been investigated. It was found that fucoidans inhibited α-glucosidase differently, depending on the algal species from which it was extracted and the algae’s season of harvest. Fucoidan extracted from A. nodosum was a more potent inhibitor of α-glucosidase, with an IC50 ranging from 0.013 to 0.047mg/mL, than the inhibition by fucoidan extracted from F. vesiculosus (IC50=0.049mg/mL). In contrast, fucoidan extracted from F. vesiculosus did not inhibit α-amylase activity, while fucoidan from A. nodosum decreased α-amylase activity by 7–100% at 5mg/mL depending upon the algae harvest period. An IC50 of 0.12–4.64mg/mL for fucoidan from A. nodosum was found for the α-amylase inhibition. The ability of fucoidan to inhibit α-amylase and α-glucosidase thus varies according to the algae species and harvest period. A. nodosum is more suitable than F. vesiculosus as a source of fucoidan to inhibit α-amylase and α-glucosidase activities. Their potential benefits towards Type 2 diabetes management should be further investigated.