Details

Autor(en) / Beteiligte

• MAEDA, Hayato
• YAMAZAKI, Mao
• KATAGATA, Yohtaro

Titel

Kuromoji (Lindera umbellata) Essential Oil Inhibits LPS-Induced Inflammation in RAW 264.7 Cells

Ist Teil von

• Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (3), p.482-486

Ort / Verlag

England: Japan Society for Bioscience, Biotechnology, and Agrochemistry

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Kuromoji (Lindera umbellata) essential oil (KEO) has long been used in Japan as a traditional medicine. It contains linalool (C10H18O), a naturally occurring small terpenoid. For this study, we investigated the anti-inflammatory effect of KEO in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Mouse macrophage-like RAW 264.7 cells were stimulated with LPS. Then they were treated with 25 or 50 µg/mL of KEO for 24 h. KEO suppressed LPS-induced pro-inflammatory cytokine production such as that of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in a dose-dependent manner. In addition, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression and protein levels were suppressed by treatment with KEO cells. In addition, by treatment with 25 or 50 µg/mL of linalool showed the same anti-inflammatory effect. The results suggest that KEO and linalool can be regarded as a natural resource for use in anti-inflammatory therapeutic products.