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A novel protein Jpk induces bacterial cell death through reactive oxygen species
Ist Teil von
Gene, 2012-08, Vol.504 (2), p.274-278
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2012
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
Jpk, a trans-acting regulatory factor associating with the position-specific regulatory element of Hoxa-7, has been reported to induce cell death in both prokaryotic and eukaryotic cells upon overexpression. The N- and C-terminal deleted variants of Jpk were constructed and then the toxicity of each construct was analyzed by checking the viability of the cells and the concomitant morphological changes through electron microscopy following the expression. The N-terminus of Jpk harboring transmembrane domain seemed to be more toxic to bacterial cell than C-terminus and the morphology of bacterial cells expressing N-terminal Jpk was similar to that induced by full length Jpk. The toxicity caused by Jpk protein in bacterial cell was through the production of ROS, which was decreased by an antioxidant (DTT) in a concentration dependent manner. The finding described in this study provides valuable clues on the relationship between Jpk toxicity and ROS generation.
► Jpk induced E. coli cell death upon expression. ► N-term half of Jpk (AA 1–90) was more toxic than C-term in aerobic condition. ► Jpk-induced bacterial cell death was mediated through the ROS. ► DTT, an antioxidant, rescued the bacterial cells expressing Jpk.