Head & neck, 2012-10, Vol.34 (10), p.1480-1491
2012
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- Autor(en) / Beteiligte
- Titel
- UM-SCC-104: A New human papillomavirus-16-positive cancer stem cell-containing head and neck squamous cell carcinoma cell line
- Ist Teil von
- Head & neck, 2012-10, Vol.34 (10), p.1480-1491
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2012
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Background Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan‐squamous cell carcinoma‐104 (UM‐SCC‐104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs). Methods Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods. Results UM‐SCC‐104 has a unique genotype, contains HPV‐16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(−) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(−) cells did not produce tumors. Conclusion UM‐SCC‐104, a new HPV‐16, CSC‐containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs. © 2011 Wiley Periodicals, Inc. Head Neck, 2011
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1043-3074eISSN: 1097-0347DOI: 10.1002/hed.21962Titel-ID: cdi_pubmed_primary_22162267
- Format
- –
- Schlagworte
- ALDH, Animals, Biological and medical sciences, Biopsy, Needle, cancer stem cells, Carcinoma, Squamous Cell - genetics, Carcinoma, Squamous Cell - pathology, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Genes, p53 - genetics, Genotype, head and neck cell lines, Head and Neck Neoplasms - genetics, Head and Neck Neoplasms - pathology, HNSCC, HPV, Human papillomavirus 16 - genetics, Human papillomavirus 16 - isolation & purification, Humans, Immunohistochemistry, In Situ Hybridization, Medical sciences, Mice, Neoplastic Stem Cells - metabolism, Neoplastic Stem Cells - pathology, Otorhinolaryngology (head neck, general aspects and miscellaneous), Otorhinolaryngology. Stomatology, Papillomavirus Infections - genetics, Papillomavirus Infections - pathology, Reverse Transcriptase Polymerase Chain Reaction - methods, RNA, Viral - metabolism, Sensitivity and Specificity, Squamous Cell Carcinoma of Head and Neck, Transplantation, Heterologous, Tumors