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Vascular-specific expression of GUS and GFP reporter genes in transgenic grapevine ( Vitis vinifera L. cv. Albariño) conferred by the EgCCR promoter of Eucalyptus gunnii
Ist Teil von
Plant physiology and biochemistry, 2011-04, Vol.49 (4), p.413-419
Ort / Verlag
Paris: Elsevier Masson SAS
Erscheinungsjahr
2011
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
In the view of the economic importance of grapevine and the increasing threaten represented by vascular diseases, transgenic grapevine with enhanced tolerance could represent an attractive opportunity. Hitherto, constitutive promoters have been used generally to study the effects of transgene expression in grapevine. Given the fact that constitutive gene expression may be harmful to the host plant, affecting plant growth and development, the use of tissue -specific promoters restricting gene expression to tissues of interest and at given developmental stages could be more appropriate. For this purpose, we decided to study in grapevine the activity of the
Eucalyptus gunnii CCR promoter that was previously reported to be vascular-preferential. We transformed grapevine with the “Sonication assisted
Agrobacterium-mediated transformation” (SAAT) method and a construct where both GUS and GFP (green fluorescent protein) marker genes were under control of the
EgCCR promoter. High GUS and GFP activities were found to be associated with the newly formed vascular tissues in stems, leaves and petioles of transformed grapevine, suggesting a preferential activity of the
EgCCR promoter in the vascular tissues of grapevine. These results suggest the tissue-specificity of this promoter from eucalyptus is conserved in grapevine and that it could be used to drive expression of defense genes in order to enhance resistance against vascular pathogens.
► The results of the present study constitute the first report on the transformation of a grapevine with the binary vector system p35SGUSint-GFP fusion gene under a vascular-specific promoter. ► We transformed grapevine microshoots using the SAAT method with a construct harboring two marker genes (GUS and GFP) under the control of the
EgCCR promoter. ► Our results strongly suggest that the
EgCCR promoter could be used to drive expression of genes (i.e. defense genes) preferentially in vascular tissues of grapevine.