Details

Autor(en) / Beteiligte

• Liu, Bin
• Zhang, Lida
• Zhu, Xinna
• Shi, Chunlei
• Chen, Jing
• Liu, Weibing
• He, Xiaohua
• Shi, Xianming

Titel

PCR identification of Salmonella serogroups based on specific targets obtained by comparative genomics

Ist Teil von

• International journal of food microbiology, 2011-01, Vol.144 (3), p.511-518

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2011

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Comparative genomic approaches provide abundant information to reveal the diversity among Salmonella serogroups. In a local genomic sequence database, twenty-five Salmonella whole genomic sequences were divided into 6 (A, B, C1, C2, D and others) serogroups for mining the DNA fragments specific for serogroups A through D. For each serogroup, a reference sequence was selected and split into 1000-bp fragments in silico to align against all the other genomic sequences to obtain one or more serogroup-specific fragments. As a result, 2, 6, 7, 10, and 7 specific fragments were found for A, B, C1, C2 and D serogroups, respectively. Specific primer sets targeting these DNA fragments were designed for multiplex PCR assays identifying 21 Salmonella standard strains and 86 additional food isolates. The PCR results demonstrated good agreement with those from Salmonella serotyping. This means that the PCR assay may be able to identify 5 (A, B, C1, C2 and D) Salmonella serogroups and elucidate differences among them. Based on the gene annotations, the 32 serogroup-specific fragments were divided into 3 categories (membrane protein genes, rfb gene clusters, and fimbrial genes). Each gene from these three groups was conserved within the serogroup and was closely correlated with phenotypic characterization. This finding implies that these genes, which are associated with sugar synthesis and metabolism or glycosyl and O-actetyl transfer, impart the differences among Salmonella serogroups. ►Comparative genomic tools are useful for identifying DNA fragments specific to Salmonella serogroups. ►This novel multiplex PCR could correctly identify Salmonella serogroups A, B, C1, C2, and D. ►A total 32 DNA fragments were identified, which contain 34 conserved genes specific to Salmonella serogroups A through D. ►Most of 34 genes are probably associated with traits that confer specific serotypic chraracteristics to Salmonella serogroups.