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Evaluation of selected South African medicinal plants for inhibitory properties against human immunodeficiency virus type 1 reverse transcriptase and integrase
Ist Teil von
Journal of ethnopharmacology, 2005-05, Vol.99 (1), p.83-91
Ort / Verlag
Shannon: Elsevier Ireland Ltd
Erscheinungsjahr
2005
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
Seventeen aqueous and methanol extracts from nine South African medicinal plants, ethnobotanically selected, were screened for inhibitory properties against HIV-1 reverse transcriptase (RT). Isolated compounds were additionally evaluated on HIV-1 integrase (IN). The strongest inhibition against the RNA-dependent-DNA polymerase (RDDP) activity of RT was observed with the methanol extract of the stem-bark of
Peltophorum africanum Sond. (Fabaceae) (IC
50 3.5
μg/ml), while the methanol extract of the roots of
Combretum molle R.Br. ex G. Don (Combretaceae) was the most inhibitory on the ribonuclease H (RNase H) activity (IC
50 9.7
μg/ml). The known compounds bergenin and catechin, and a red coloured gallotannin composed of
meta-depside chains of gallic and protocatechuic acids esterified to a 1-
O-isobutyroly-β-
d-glucopyranose core, were isolated from the methanol extract of the roots and stem-bark of
Peltophorum africanum. The gallotannin inhibited the RDDP and RNase H functions of RT with IC
50 values of 6.0 and 5.0
μM, respectively, and abolished the 3′-end processing activity of IN at 100
μM. Catechin showed no effect on RT but had a moderate activity on HIV-1 IN. Bergenin was inactive on both enzymes. The aqueous and methanol extracts were non-toxic in a HeLaP4 cell line at a concentration of 400
μg/ml.