Details

Autor(en) / Beteiligte

• Nagaraja, Padmarajaiah
• Shrestha, Ashwinee
• Shivakumar, Anantharaman
• Gowda, Avinash

Titel

Use of N, N-diethyl-p-phenylenediamine sulphate for the spectrophotometric determination of some phenolic and amine drugs

Ist Teil von

• Acta pharmaceutica (Zagreb, Croatia), 2010-06, Vol.60 (2), p.217-227

Ort / Verlag

Croatia: Versita

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Spectrophotometric methods are proposed for the determination of drugs containing a phenol group [salbutamol sulphate (SLB), ritodrine hydrochloride (RTD), isoxsuprine hydrochloride (IXP)] and drugs containing an aromatic amine group [dapsone hydrochloride (DAP), sulfamethoxazole (SFM), and sulfadiazine (SFD)] in pharmaceutical dosage forms. The methods are based on coupling of N, N-diethyl-p-phenylenediamine sulphate with the drugs in the presence of KIO4 to give a green colored product (λmax at 670 nm) and a red colored product (λmax at 550 nm), respectively. Linear relationships with good correlation coefficients (0.9986-0.9996) were found between absorbance and the corresponding concentration of drugs in the range 1-7, 2-22, 1-17, 1.5-12, 2-25, and 2-21 μg mL-1 for SLB, RTD, IXP, DAP, SFM and SFD, respectively. Variable parameters such as temperature, reaction time and concentration of the reactants have been analyzed and optimized. The RSD of intra-day and inter-day studies was in the range of 0.2-1.0 and 0.4-1.0%, respectively. No interference was observed from common pharmaceutical adjuvants. The reliability and performance of the proposed methods was validated statistically; the percentage recovery ranged from 99.5 ± 0.1 to 99.9 ± 0.3%. Limits of detection were 0.14, 0.21, 0.51, 0.44, 0.33 and 0.37 μg mL-1 for SLB, RTD, IXP, DAP, SFM, and SFD, respectively. U radu je predložena spektrofotometrijska metoda za određivanje lijekova s fenolnom skupinom [salbutamol sulfat (SLB), ritodrin hidroklorid (RTD), izoksuprin hidroklorid (IXP)] i lijekova s aromatskom amino skupinom [dapson hidroklorid (DAP), sulfametoksazol (SFM) i sulfadiazin (SFD)] u farmaceutskim dozirnim pripravcima. Metode se temelje na reakciji ljekovitih tvari s N, N-dietil-p-fenilendiamin sulfatom u prisutnosti KIO4, pri čemu nastaje zeleni (λmax pri 670 nm), odnosno crveni produkt (λmax pri 550 nm). Apsorbancije linerano ovise o koncentrancijama lijekova uz visok koeficijent korelacije (0,9986-0,9996) u koncentracijskom području 1-7, 2-22, 1-17, 1,5-12, 2-25 i 2-21 μg mL-1 za SLB, RTD, IXP, DAP, SFM i SFD. Analizirani su i optimirani promjenjivi parametri kao što su temperatura, reakcijsko vrijeme i koncentracija reaktanata. Repetibilnost i intermedijarna preciznost iznosile su 0,2-1,0, odnosno 0,4-1,0%. Nije primjećena nikakva interferencija s uobičajenim farmaceutskim pomoćnim sredstvima. Pouzdanost i izvedbene značajke predložene metode validirane su statistički. Povrat analitičke metode bio je od 99,5 ± 0,1 do 99,9 ± 0,3%. Granice detekcije bile su 0,14, 0,21, 0,51, 0,44, 0,33 i 0,37 μg mL-1 za SLB, RTD, IXP, DAP, SFM, odnosno SFD.