Details

Autor(en) / Beteiligte

• Meyer-König, Ursula
• Haberland, Michael
• von Laer, Dorothee
• Haller, Otto
• Hufert, Frank T.

Titel

Intragenic Variability of Human Cytomegalovirus Glycoprotein B in Clinical Strains

Ist Teil von

• The Journal of infectious diseases, 1998-05, Vol.177 (5), p.1162-1169

Ort / Verlag

Chicago, IL: The University of Chicago Press

Erscheinungsjahr

1998

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Human cytomegalovirus (HCMV) strains can be classified into four glycoprotein B (gB) genotypes, and there has been evidence of differences in viral virulence. In this study, intragenic variability of HCMV gB strains was analyzed. The gB gene was amplified by nested polymerase chain reaction using samples from immunosuppressed patients. The genotype of fragments corresponding to the cleavage site of gB was determined by restriction fragment analysis; fragments corresponding to the N- and C-termini (gBn and gBc) were sequenced and compared with published sequences. At the cleavage site, the four known genotypes were found. Typing revealed four major genotypes at the N-terminus and two at the C-terminus. In 22 of 44 strains, the gB type determined at the cleavage site was different from the gBn or gBc type (or either), indicating that intragenic variability within the gB gene occurs frequently.