Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Veterinary immunology and immunopathology, 1994-10, Vol.43 (1), p.13-18
1994

Details

Autor(en) / Beteiligte
Titel
Porcine T-cell receptors: molecular and biochemical characterization
Ist Teil von
  • Veterinary immunology and immunopathology, 1994-10, Vol.43 (1), p.13-18
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
1994
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
  • Two subclasses of CD3 associated T-cell receptors (TcR) have been described so far, consisting of either an α and β chain (TcR αβ) or a γ and δ chain (TcR γδ). Of the two subclasses, the TcR αβ is the one predominantly expressed on peripheral T lymphocytes of humans and rodents. TcR γδ T lymphocytes represent only a minor subset in these species. Among all mammalian species studied so far, swine showed the most diversified composition of the T-lymphocyte population characterized by the expression of CD4 and CD8 differentiation antigens. Besides CD4 +CD8 − and CD4 −CD8 + T lymphocytes, CD4 +CD8 + and CD4 −CD8 − T lymphocytes are prominent in the extrathymic T-lymphocyte compartment. Because of the lack of specific monoclonal antibodies (mAb), to date the porcine TcR can only be characterized with biochemical and molecular biological methods. TcR on porcine peripheral blood T lymphocytes with the phenotype CD4 + and/or CD8 + are characterized as 46–48 kDa R heterodimers which were supposed to represent the porcine TcR αβ. Biochemical analyses of the CD4 −CD8 − T lymphocytes revealed three distinct TcR γδ; all are characterized by a 40 kDa δ chain but differed in their γ chains. One γ chain with a molecular mass of 38 kDa R is preferentially expressed on CD4 −CD8 − T lymphocytes derived from peripheral blood; another chain with molecular mass of 37 kDa R is evenly distributed between CD4 −CD8 − T lymphocytes from blood and lymphoid tissues. A third γ chain characterized by a molecular mass of 46 kDa R is expressed on CD2 +CD4 −CD8 − T lymphocytes enriched in lymphoid tissues. The molecular characterization of porcine TcR-constant regions revealed the existence of one α, possibly two β, at least three γ and one δ TcR-constant region isotypes, which show very high homology on amino acid level with the corresponding TcR chains of other species.

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX