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Identification of the Mitochondrial GTP/GDP Transporter in Saccharomyces cerevisiae
Ist Teil von
The Journal of biological chemistry, 2004-05, Vol.279 (20), p.20850-20857
Ort / Verlag
United States: American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
2004
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The genome of Saccharomyces cerevisiae contains 35 members of a family of transport proteins that, with a single exception, are found in the inner membranes of
mitochondria. The transport functions of the 16 biochemically identified mitochondrial carriers are concerned with shuttling
substrates, biosynthetic intermediates, and cofactors across the inner membrane. Here the identification and functional characterization
of the mitochondrial GTP/GDP carrier (Ggc1p) is described. The ggc1 gene was overexpressed in bacteria. The purified protein was reconstituted into liposomes, and its transport properties and
kinetic parameters were characterized. It transported GTP and GDP and, to a lesser extent, the corresponding deoxynucleotides
and the structurally related ITP and IDP by a counter-exchange mechanism. Transport was saturable with an apparent K m of 1 μ m for GTP and 5 μ m for GDP. It was strongly inhibited by pyridoxal 5â²-phosphate, bathophenanthroline, tannic acid, and bromcresol purple but
little affected by the inhibitors of the ADP/ATP carrier carboxyatractyloside and bongkrekate. Furthermore, in contrast to
the ADP/ATP carrier, the Ggc1p-mediated GTP/GDP heteroexchange is H + -compensated and thus electroneutral. Cells lacking the ggc1 gene had reduced levels of GTP and increased levels of GDP in their mitochondria. Furthermore, the knock-out of ggc1 results in lack of growth on nonfermentable carbon sources and complete loss of mitochondrial DNA. The physiological role
of Ggc1p in S. cerevisiae is probably to transport GTP into mitochondria, where it is required for important processes such as nucleic acid and protein
synthesis, in exchange for intramitochondrially generated GDP.