Details

Autor(en) / Beteiligte

• Kawamura, Hidenobu
• Tomozoe, Yusuke
• Akagi, Tadayuki
• Kamei, Daisuke
• Ochiai, Michiru
• Yamada, Michiyuki

Titel

Identification of the Nucleocytoplasmic Shuttling Sequence of Heterogeneous Nuclear Ribonucleoprotein D-like Protein JKTBP and Its Interaction with mRNA

Ist Teil von

• The Journal of biological chemistry, 2002-01, Vol.277 (4), p.2732-2739

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• JKTBP proteins are related to a family of heterogeneous nuclear ribonucleoproteins (hnRNPs) that function in mRNA biogenesis and mRNA metabolism. JKTBP proteins constituted of isoforms 1, 2, and 1Δ6 are localized in the nucleus. We show that the dominant form JKTBP1 shuttles between the nucleus and the cytoplasm and interacts with mRNA. Immunofluorescence microscopy and immunoblotting of the subcellular fractions and overexpression of JKTBP tagged with green fluorescent protein indicated that JKTBP1 and JKTBP1Δ6, but not JKTBP2, accumulate in the cytoplasm upon polymerase II transcription inhibition. After release from inhibition, the return of accumulated cytoplasmic JKTBP to the nucleus was temperature-dependent. In heterokaryons, green fluorescent protein-tagged JKTBP1 and JKTBP1Δ6 migrated from the HeLa nucleus to the mouse nucleus, but JKTBP2 did not. Using various JKTBP deletion mutants, the 25-residue C-terminal tail was identified as a shuttling sequence like M9. It is conserved in the C-terminal tails of hnRNP D/AUF1 and type A/B hnRNP/ABBP-1. Analysis of its sequence-specific interacting protein indicated that JKTBP nuclear import is mediated by the receptor transportin 1/karyopherin β2. UV cross-linking revealed the increased occurrence of JKTBP1 directly interacting with poly(A)+ RNA in the cytoplasm following actinomycin D treatment. We discuss a role of JKTBP in mRNA nuclear export.