Details

Autor(en) / Beteiligte

• Lumbierres, Maria
• Palomo, Jose M.
• Kragol, Goran
• Roehrs, Sonja
• Müller, Oliver
• Waldmann, Herbert

Titel

Solid-Phase Synthesis of Lipidated Peptides

Ist Teil von

• Chemistry : a European journal, 2005-12, Vol.11 (24), p.7405-7415

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2005

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• A new flexible and efficient methodology for the solid‐phase synthesis of lipidated peptides has been developed. The approach is based on the use of previously synthesized building blocks and overcomes the limitations of previously reported methods, since long doubly lipidated peptides can be synthesized by using this route. Furthermore, it was thus possible to prepare a large number of N‐ and H‐Ras peptides bearing a wide range of reporter and/or linking groups—efficient tools for the investigation of biological processes. In terms of efficiency and flexibility this solid‐phase method is superior to the solution‐phase synthesis. It gives pure peptides in multimilligram amounts within a much shorter time and with superior overall yield. A new flexible and efficient methodology, based on the use of lipidated amino acid building blocks, has been developed for the solid‐phase synthesis of lipidated peptides incorporating different linking and reporter groups (see scheme).