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Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis
Ist Teil von
Journal of medical microbiology, 2007-11, Vol.56 (11), p.1467-1473
Ort / Verlag
England: Soc General Microbiol
Erscheinungsjahr
2007
Link zum Volltext
Quelle
EZB-FREE-00999 freely available EZB journals
Beschreibungen/Notizen
1 Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan
2 Yodogawa Christian Hospital, Osaka, Japan
3 Microbiological Department, Kobe Institute of Health, Hyogo, Japan
4 Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
Correspondence Wataru Yamazaki-Matsune wataru{at}iph.pref.osaka.jp
Received 26 April 2007
Accepted 9 July 2007
A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli , Campylobacter fetus , Campylobacter hyointestinalis subsp. hyointestinalis , Campylobacter jejuni , Campylobacter lari and Campylobacter upsaliensis . The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.
Present address : Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences are AB301955–AB301967.