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Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans
Science (American Association for the Advancement of Science), 2007-01, Vol.315 (5809), p.241-244
2007

Details

Autor(en) / Beteiligte
Titel
Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans
Ist Teil von
  • Science (American Association for the Advancement of Science), 2007-01, Vol.315 (5809), p.241-244
Ort / Verlag
Washington, DC: American Association for the Advancement of Science
Erscheinungsjahr
2007
Link zum Volltext
Quelle
American Association for the Advancement of Science
Beschreibungen/Notizen
  • RNA interference (RNAi) is a phylogenetically widespread gene-silencing process triggered by double-stranded RNA. In plants and Caenorhabditis elegans, two distinct populations of small RNAs have been proposed to participate in RNAi: "Primary siRNAs" (derived from DICER nuclease-mediated cleavage of the original trigger) and "secondary siRNAs" [additional small RNAs whose synthesis requires an RNA-directed RNA polymerase (RdRP)]. Analyzing small RNAs associated with ongoing RNAi in C. elegans, we found that secondary siRNAs constitute the vast majority. The bulk of secondary siRNAs exhibited structure and sequence indicative of a biosynthetic mode whereby each molecule derives from an independent de novo initiation by RdRP. Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular messenger RNAs by RdRP activity may have key roles in cellular regulation.
Sprache
Englisch
Identifikatoren
ISSN: 0036-8075
eISSN: 1095-9203
DOI: 10.1126/science.1132839
Titel-ID: cdi_proquest_miscellaneous_68421034
Format
–
Schlagworte
Animals, Antisense RNA, Biochemistry, Biological and medical sciences, Caenorhabditis elegans, Caenorhabditis elegans - genetics, Caenorhabditis elegans - metabolism, Caenorhabditis elegans Proteins - metabolism, Cellular biology, Cloning, Molecular, Double stranded RNA, Fundamental and applied biological sciences. Psychology, Gels, Gene silencing, Genetics, Insects, Ligation, Messenger RNA, MicroRNA, MicroRNAs - metabolism, Models, Genetic, Molecular and cellular biology, Molecular genetics, Phosphates, Ribonucleic acid, RNA, RNA Interference, RNA Replicase - metabolism, RNA, Antisense - biosynthesis, RNA, Antisense - chemistry, RNA, Antisense - metabolism, RNA, Double-Stranded - genetics, RNA, Double-Stranded - metabolism, RNA, Helminth - chemistry, RNA, Helminth - genetics, RNA, Helminth - metabolism, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNA, Small Interfering - biosynthesis, RNA, Small Interfering - chemistry, RNA, Small Interfering - metabolism, Small interfering RNA, Transcription. Transcription factor. Splicing. Rna processing

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