Science (American Association for the Advancement of Science), 2009-09, Vol.325 (5946), p.1388-1390
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Acidic Matrix Protein, Pif, Is a Key Macromolecule for Nacre Formation
- Ist Teil von
- Science (American Association for the Advancement of Science), 2009-09, Vol.325 (5946), p.1388-1390
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2009
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- The mollusk shell is a hard tissue consisting of calcium carbonate crystals and an organic matrix. The nacre of the shell is characterized by a stacked compartment structure with a uniformly oriented c axis of aragonite crystals in each compartment. Using a calcium carbonate-binding assay, we identified an acidic matrix protein, Pif, in the pearl oyster Pinctada fucata that specifically binds to aragonite crystals. The Pif complementary DNA (cDNA) encoded a precursor protein, which was posttranslationally cleaved to produce Pif 97 and Pif 80. The results from immunolocalization, a knockdown experiment that used RNA interference, and in vitro calcium carbonate crystallization studies strongly indicate that Pif regulates nacre formation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1173793Titel-ID: cdi_proquest_miscellaneous_67646015
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Aragonite, Biochemistry. Physiology. Immunology, Biological and medical sciences, Calcification, Physiologic, Calcium Carbonate - chemistry, Calcium Carbonate - metabolism, Calcium carbonates, Chemical compounds, Chitin, Complementary DNA, Crystallization, Crystals, DNA, Complementary, Epithelial Cells - metabolism, Fundamental and applied biological sciences. Psychology, Glass, Hydrogen-Ion Concentration, Immunohistochemistry, Invertebrates, Molecular Sequence Data, Molecules, Mollusca, Mollusks, Nanoparticles, Pearls, Physiology. Development, Pinctada - chemistry, Pinctada - metabolism, Pinctada fucata, Protein Precursors - genetics, Protein Precursors - metabolism, Protein Processing, Post-Translational, Proteins, Proteins - chemistry, Proteins - genetics, Proteins - metabolism, RNA Interference