Details

Autor(en) / Beteiligte

• Le Vay, Kristian
• Song, Emilie Yeonwha
• Ghosh, Basusree
• Tang, T.‐Y. Dora
• Mutschler, Hannes

Titel

Enhanced Ribozyme‐Catalyzed Recombination and Oligonucleotide Assembly in Peptide‐RNA Condensates

Ist Teil von

• Angewandte Chemie (International ed.), 2021-12, Vol.60 (50), p.26096-26104

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The ability of RNA to catalyze RNA ligation is critical to its central role in many prebiotic model scenarios, in particular the copying of information during self‐replication. Prebiotically plausible ribozymes formed from short oligonucleotides can catalyze reversible RNA cleavage and ligation reactions, but harsh conditions or unusual scenarios are often required to promote folding and drive the reaction equilibrium towards ligation. Here, we demonstrate that ribozyme activity is greatly enhanced by charge‐mediated phase separation with poly‐L‐lysine, which shifts the reaction equilibrium from cleavage in solution to ligation in peptide‐RNA coaggregates and coacervates. This compartmentalization enables robust isothermal RNA assembly over a broad range of conditions, which can be leveraged to assemble long and complex RNAs from short fragments under mild conditions in the absence of exogenous activation chemistry, bridging the gap between pools of short oligomers and functional RNAs. In solution, a prebiotically plausible ribozyme formed from short oligonucleotides reversibly cleaves an RNA substrate. However, in the presence of poly‐L‐lysine, phase separation occurs owing to charge interactions between the oppositely charged RNA and peptide. The resulting compartments greatly enhance ribozyme activity, driving the reaction equilibrium towards the ligation of long and complex RNA in mild conditions.