Details

Autor(en) / Beteiligte

• Yokoi, Ken-ji
• Tsutsui, Sosyu
• Arakawa, Gen-ya
• Takaba, Masakazu
• Fujii, Koichi
• Kaneko, Satoshi

Titel

Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691

Ist Teil von

• Bioscience, biotechnology, and biochemistry, 2021-07, Vol.85 (8), p.1830-1838

Erscheinungsjahr

2021

Link zum Volltext

Beschreibungen/Notizen

• ABSTRACT Information about the inulosucrase of nonlactic acid bacteria is scarce. We found a gene encoding inulosucrase (inuBK) in the genome of the Gram-positive bacterium Alkalihalobacillus krulwichiae JCM 11691. The inuBK open reading frame encoded a protein comprising 456 amino acids. We expressed His-tagged InuBK in culture medium using a Brevibacillus system. The optimal pH and temperature of purified InuBK were 7.0-9.0 and 50-55 °C, respectively. The findings of high-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy, and high-performance size-exclusion chromatography with multiangle laser light scattering showed that the polysaccharide produced by InuBK was an inulin with a molecular weight of 3806, a polydispersity index (PI) of 1.047, and fructosyl chain lengths with 3-27 degrees of polymerization. The size of InuBK was smaller than commercial inulins, and the PI of the inulin that it produced was lower.