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Characterization of MYB35 regulated methyl jasmonate and wound responsive Geraniol 10-hydroxylase-1 gene from Bacopa monnieri
Ist Teil von
Planta, 2021-05, Vol.253 (5), p.89-89, Article 89
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2021
Link zum Volltext
Quelle
SpringerLink
Beschreibungen/Notizen
Main conclusion
BmG10H-1
transcript from
B. monnieri
was functionally active.
BmG10H-1
promoter drives GUS activity in response to MeJA and wounding. BmMYB35 regulates
BmG10H-1
transcript by binding to its promoter.
Geraniol 10-hydroxylase (G10H) is one of the important regulatory cytochrome P450 monooxygenase, which is involved in the biosynthesis of monoterpene alkaloids. However, G10H is not characterized at the enzymatic or at the regulatory aspect in
B. monnieri
. In the present study, we have identified two transcripts of
BmG10H
(
BmG10H-1
and
BmG10H-2
) and characterized the methyl jasmonate (MeJA) and wound responsive
BmG10H-1
transcript from
B. monnieri. BmG10H-1
showed induced expression after 3 h of MeJA and wounding treatment in the shoot
.
Yeast purified recombinant BmG10H-1 protein is enzymatically active, having V
max
of 0.16 µMsec
−1
μg
−1
protein and catalyzes the hydroxylation of geraniol to 10-hydroxy geraniol. The
BmG10H-1
promoter was isolated by using the genome walking method.
BmG10H-1
promoter can drive GUS expression in transgenic
Arabidopsis thaliana
. GUS activity of MeJA and wound-treated
Arabidopsis
seedlings were found to be increased as compared to the control untreated seedlings, whereas no GUS activity was found in deleted MeJA responsive and W-box
cis
-elements. This shows that the
BmG10H-1
promoter contains functional MeJA (TGACG) and wound responsive (TGACCT)
cis
-elements. Further, shoot specific and MeJA responsive recombinant BmMYB35 protein was purified, which binds with the MYB recognition
cis
-element (TGGTTA) present in the
BmG10H-1
promoter and transcriptionally activates the reporter gene in yeast. In conclusion, the characterization of MeJA and wound responsive
BmG10H-1
provides novel information about its transcriptional regulation by binding with MYB transcription factor in
B. monnieri
.