Details

Autor(en) / Beteiligte

• Pan, Xuewei
• Tang, Mi
• You, Jiajia
• Liu, Fei
• Sun, Changhao
• Osire, Tolbert
• Fu, Weilai
• Yi, Ganfeng
• Yang, Taowei
• Yang, Shang-Tian
• Rao, Zhiming
• Zhou, Ning-Yi

Titel

Regulator RcsB Controls Prodigiosin Synthesis and Various Cellular Processes in Serratia marcescens JNB5-1

Ist Teil von

• Applied and environmental microbiology, 2021-01, Vol.87 (2), p.1

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Prodigiosin (PG), a red linear tripyrrole pigment normally secreted by , has received attention for its reported immunosuppressive, antimicrobial, and anticancer properties. Although several genes have been shown to be important for prodigiosin synthesis, information on the regulatory mechanisms behind this cellular process remains limited. In this work, we identified that the transcriptional regulator RcsB encoding gene ( ) negatively controlled prodigiosin biosynthesis in Disruption of conferred a remarkably increased production of prodigiosin. This phenotype corresponded to negative control of transcription of the prodigiosin-associated operon by RcsB, probably by binding to the promoter region of the prodigiosin synthesis positive regulator FlhDC. Moreover, using transcriptomics and further experiments, we revealed that RcsB also controlled some other important cellular processes, including swimming and swarming motilities, capsular polysaccharide production, biofilm formation, and acid resistance (AR), in Collectively, this work proposes that RcsB is a prodigiosin synthesis repressor in and provides insight into the regulatory mechanism of RcsB in cell motility, capsular polysaccharide production, and acid resistance in RcsB is a two-component response regulator in the Rcs phosphorelay system, and it plays versatile regulatory functions in However, information on the function of the RcsB protein in bacteria, especially in , remains limited. In this work, we illustrated experimentally that the RcsB protein was involved in diverse cellular processes in , including prodigiosin synthesis, cell motility, capsular polysaccharide production, biofilm formation, and acid resistance. Additionally, the regulatory mechanism of the RcsB protein in these cellular processes was investigated. In conclusion, this work indicated that RcsB could be a regulator for prodigiosin synthesis and provides insight into the function of the RcsB protein in .