Details

Autor(en) / Beteiligte

• Nayek, Subhayu
• De Silva, Imesha W.
• Aguilar, Roberto
• Lund, Amie K.
• Verbeck, Guido F.

Titel

Toxicological alterations induced by subacute exposure of silver nanoparticles in Wistar rats

Ist Teil von

• Journal of applied toxicology, 2021-06, Vol.41 (6), p.972-986

Ort / Verlag

England: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Silver nanoparticles (AgNPs) have become crucial players in the field of medicine and various other industries. AgNPs have a wide array of applications, which includes production of electronic goods, cosmetics, synthesis of dyes, and printing inks, as well as targeted delivery of drugs to specialized cells inside the body. Even though humans readily come in contact with these particles, the organ‐specific accumulation and resulting mechanisms of toxicity induced by inhaled AgNPs are still under investigation. The goal of this study was to determine the organ distribution of inhaled AgNPs and investigate the resulting systemic toxicity. To do this, male Wistar rats were exposed by inhalation to AgNPs for 4 hr/day (200 parts per billion/day) for five consecutive days. The nanoparticles were generated using a laser ablation technique using a soft‐landing ion mobility (SLIM) instrument. Inductively coupled plasma mass spectrometric (ICP‐MS) analysis showed organ‐specific accumulation of the nanoparticles, with the highest concentration present in the lungs, followed by the liver and kidneys. Nanoparticle distribution was characterized in the organs using scanning electron microscopy (SEM) and matrix‐assisted laser desorption/ionization mass spectrometric (MALDI‐MS) imaging. Bone marrow cytotoxicity assay of the cells from the femur of rats showed micronuclei formation and signs of cellular cytotoxicity. Moreover, rats displayed increased levels of circulating lactate and glutathione disulphide (GSSG), as determined by liquid chromatography‐mass spectrometry (LC‐MS) analysis. Collectively, our observations suggest that inhaled subacute exposure to AgNP results in accumulation of AgNPs in the lungs, liver, and kidneys, preferentially, as well as mediates induced systemic toxicity. This study investigated tissue localization and markers of systemic toxicity following subacute inhalation exposure to silver nanoparticles (AgNPs: 200 ppb for 4 hr/day for 5 days) in male Wistar rats. Mass spectrometric and microscopic techniques were used to localize and quantify AgNPs in organ tissues, and to characterize their size distribution. AgNP‐exposure was associated with elevated levels of blood lactate and plasma glutathione disulphide, indicating systemic oxidative stress, and a bone marrow cytotoxicity assay revealed increased signs of cellular toxicity.