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Alteration of Streptococcus salivarius in Buccal Mucosa of Oral Lichen Planus and Controlled Clinical Trial in OLP Treatment
Ist Teil von
Probiotics and antimicrobial proteins, 2020-12, Vol.12 (4), p.1340-1348
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2020
Link zum Volltext
Quelle
SpringerNature Journals
Beschreibungen/Notizen
Oral lichen planus (OLP) is a T cell-mediated common chronic inflammatory mucosal disease, with limited therapies available for long-term use. Previous study showed that ratio of genus
Streptococcus
decreased significantly in OLP patients when compared with controls. Buccal cotton swab samples of 43 OLP patients and 48 healthy individuals were collected for real-time quantitative polymerase chain reaction (RT-PCR) to investigate relative abundance alteration of
Streptococcus salivarius
in OLP lesions. Bacterial supernatants of
S. salivarius
ATCC® BAA-2593™ were collected by centrifugation and added to HSC-3 cells, and quantitative analysis of expression of IL-1β, IL-6, IL-8, and TNF-α in the HSC-3 cells was determined by RT-PCR. Then, a randomized, non-blinded, controlled study was conducted. Forty patients with symptomatic OLP were randomly allocated into two groups and received topical treatment of 0.1% triamcinolone acetonide dental paste (group A) and
S. salivarius
K12 lozenge (group B), respectively, for 4 weeks. Sign scores, visual analogue scale (VAS), and adverse reactions were recorded. Relative abundance of
S. salivarius
in the OLP group was lower than that of control group (
P
< 0.05). After treated with 0.1% supernatants of
S. salivarius
ATCC® BAA-2593™, the expression level of IL-6 in the HSC-3 cells significantly reduced (
P
< 0.001), while IL-1β, IL-8, and TNF- α showed a decreasing tendency (
P
> 0.05). There was significant reduction in sign scores and VAS scores in both groups after the 4-week treatment, with no significant difference between two groups. No adverse reaction was observed.
S. salivarius
might maintain local immune balance by inhibiting the NF-κB pathway. Topical application of
Streptococcus salivarius
K12 seemed to be effective in treatment of symptomatic OLP, especially with promising potential in long-term use. More detailed clinical studies with long follow-up period and standardized usage/dosage are expected to acquire definite conclusions.