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On‐Demand Electrochemical Epoxidation in Nano‐Electrospray Ionization Mass Spectrometry to Locate Carbon–Carbon Double Bonds
Ist Teil von
Angewandte Chemie (International ed.), 2020-01, Vol.59 (1), p.209-214
Auflage
International ed. in English
Ort / Verlag
Germany: Wiley Subscription Services, Inc
Erscheinungsjahr
2020
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Reported here is the first on‐demand electrochemical epoxidation incorporated into the standard nano‐electrospray ionization mass spectrometry (nanoESI‐MS) workflow for double‐bond identification. The capability lies in a novel tunable electro‐epoxidation of double bonds, where onset of the reaction can be controlled by simply tuning the spray voltage. On‐demand formation of mono‐/multiple epoxides is achieved at different voltages. The electro‐epoxidized products are then fragmented by tandem MS to generate diagnostic ions, indicating the double bond position(s). The process is completed within seconds, holding great potential for high‐throughput analysis. The rapid switch‐on/off electro‐epoxidation of a single sample, the low sample consumption, the demonstrated applicability to complex lipids containing multiple double bonds, and the advantage of not requiring extra apparatus make this method attractive for use in lipid‐related biological studies.
Scoring position: The onset of electro‐epoxidation of lipid double bonds can be controlled by simply tuning the spray voltage. The electro‐epoxidized products are then fragmented by tandem mass spectrometry to generate diagnostic ions, indicating the double‐bond position(s). On‐demand derivatization, rapid response, low sample consumption, and the advantage of not requiring extra apparatus make this method attractive for use in lipid biology.