Details

Autor(en) / Beteiligte

• Yu, Min
• Bruns, David E
• Katzmann, Jerry A
• Silverman, Lawrence M
• Murray, David L

Titel

Restricted IgG-Kappa and Free Alpha-Heavy-Chain Bands in an Asymptomatic 62-Year-Old Man

Ist Teil von

• Clinical chemistry (Baltimore, Md.), 2018-02, Vol.64 (2), p.265-268

Ort / Verlag

England: American Association for Clinical Chemistry, Inc

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• With its lower detection limit, it is more diagnostically sensitive than protein electrophoresis. Because the immunoprecipitation relies on interaction of antibody molecules with antigen, interpretation of the IFE can be complicated by several factors: 1) Under- or overdilution of serum prevents recognition of light chains. According to the protocol in our institution, for patients with a total immunoglobulin ≤2000 mg/dL, as in this patient, a 3-fold dilution of serum was used in both light-chain tracks, leaving open the possibility that a different dilution might have revealed a light-chain partner of the α-heavy chain. 2) Differing analytical performance of heavy- and lightchain reagents may lead to failure to identify light chains. Differing analytical detection limits for heavy and light chains can lead to either false-negative or false-positive results for heavy-chain disease. [...]some antisera reagents, especially anti-IgM and anti-IgA reagents, have cross-reactivities with other proteins. [...]the masses of the IgA peak and of the λ peak were identical, indicating the presence of an IgA λ M-protein. [...]the patient had a single IgGк M-protein and an intact IgAλ protein.