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Isolation and Characterization of a Human Intestinal Bacterium Eggerthella sp. AUH-JLD49s for the Conversion of (−)-3′-Desmethylarctigenin
Ist Teil von
Journal of agricultural and food chemistry, 2017-05, Vol.65 (20), p.4051-4056
Ort / Verlag
United States: American Chemical Society
Erscheinungsjahr
2017
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Arctiin is the most abundant bioactive compound contained in the Arctium lappa plant. In our previous study, we isolated one single bacterium capable of bioconverting arctigenin, an aglycone of arctiin, to 3′-desmethylarctigenin (3′-DMAG) solely. However, to date, a specific bacterium capable of producing other arctiin metabolites has not been reported. In this study, we isolated one single bacterium, which we named Eggerthella sp. AUH-JLD49s, capable of bioconverting 3′-DMAG under anaerobic conditions. The metabolite of 3′-DMAG by strain AUH-JLD49s was identified as 3′-desmethyl-4′-dehydroxyarctigenin (DMDH-AG) based on electrospray ionization mass spectrometry (ESI–MS) and 1H and 13C nuclear magnetic resonance spectroscopy. The bioconversion kinetics and bioconversion capacity of strain AUH-JLD49s were investigated. In addition, the metabolite DMDH-AG showed an inhibitory effect on cell growth of human colon cancer cell line HCT116 and human breast cancer cell line MDA-MB-231.