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Details

Autor(en) / Beteiligte
Titel
Calcium-mediated oxidative stress: a common mechanism in tight junction disruption by different types of cellular stress
Ist Teil von
  • Biochemical journal, 2017-02, Vol.474 (5), p.731-749
Ort / Verlag
England: Portland Press Ltd
Erscheinungsjahr
2017
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
  • The role of reactive oxygen species (ROS) in osmotic stress, dextran sulfate sodium (DSS) and cyclic stretch-induced tight junction (TJ) disruption was investigated in Caco-2 cell monolayers and restraint stress-induced barrier dysfunction in mouse colon Live cell imaging showed that osmotic stress, cyclic stretch and DSS triggered rapid production of ROS in Caco-2 cell monolayers, which was blocked by depletion of intracellular Ca by 1,2-bis-( -aminophenoxy)ethane- , , ', '-tetraacetic acid. Knockdown of Ca 1.3 or TRPV6 channels blocked osmotic stress and DSS-induced ROS production and attenuated TJ disruption and barrier dysfunction. -Acetyl l-cysteine (NAC) and l- -Nitroarginine methyl ester (l-NAME) blocked stress-induced TJ disruption and barrier dysfunction. NAC and l-NAME also blocked stress-induced activation of c-Jun -terminal kinase (JNK) and c-Src. ROS was colocalized with the mitochondrial marker in stressed cells. Cyclosporin A blocked osmotic stress and DSS-induced ROS production, barrier dysfunction, TJ disruption and JNK activation. Mitochondria-targeted Mito-TEMPO blocked osmotic stress and DSS-induced barrier dysfunction and TJ disruption. Chronic restraint stress in mice resulted in the elevation of intracellular Ca , activation of JNK and c-Src, and disruption of TJ in the colonic epithelium. Furthermore, corticosterone administration induced JNK and c-Src activation, TJ disruption and protein thiol oxidation in colonic mucosa. The present study demonstrates that oxidative stress is a common signal in the mechanism of TJ disruption in the intestinal epithelium by different types of cellular stress and bio behavioral stress .
Sprache
Englisch
Identifikatoren
ISSN: 0264-6021, 1470-8728
eISSN: 1470-8728
DOI: 10.1042/BCJ20160679
Titel-ID: cdi_proquest_miscellaneous_1856591316

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