Details

Autor(en) / Beteiligte

• Liu, Yansheng
• Beyer, Andreas
• Aebersold, Ruedi

Titel

On the Dependency of Cellular Protein Levels on mRNA Abundance

Ist Teil von

• Cell, 2016-04, Vol.165 (3), p.535-550

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• The question of how genomic information is expressed to determine phenotypes is of central importance for basic and translational life science research and has been studied by transcriptomic and proteomic profiling. Here, we review the relationship between protein and mRNA levels under various scenarios, such as steady state, long-term state changes, and short-term adaptation, demonstrating the complexity of gene expression regulation, especially during dynamic transitions. The spatial and temporal variations of mRNAs, as well as the local availability of resources for protein biosynthesis, strongly influence the relationship between protein levels and their coding transcripts. We further discuss the buffering of mRNA fluctuations at the level of protein concentrations. We conclude that transcript levels by themselves are not sufficient to predict protein levels in many scenarios and to thus explain genotype-phenotype relationships and that high-quality data quantifying different levels of gene expression are indispensable for the complete understanding of biological processes. Genomic information becomes translated into phenotypes via the complex and highly regulated process of gene expression and translation. Here, we review the relationship between mRNA and protein levels under various scenarios and conclude that transcript abundance by itself is not always sufficient to predict protein levels, emphasizing the complexity of the genotype-phenotype relationships.