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BibTeX
High-resolution MALDI-FT-ICR MS imaging for the analysis of metabolites from formalin-fixed, paraffin-embedded clinical tissue samples
The Journal of pathology, 2015-09, Vol.237 (1), p.123-132
Buck, Achim
Ly, Alice
Balluff, Benjamin
Sun, Na
Gorzolka, Karin
Feuchtinger, Annette
Janssen, Klaus-Peter
Kuppen, Peter JK
van de Velde, Cornelis JH
Weirich, Gregor
Erlmeier, Franziska
Langer, Rupert
Aubele, Michaela
Zitzelsberger, Horst
Aichler, Michaela
Walch, Axel
2015
Details
Autor(en) / Beteiligte
Buck, Achim
Ly, Alice
Balluff, Benjamin
Sun, Na
Gorzolka, Karin
Feuchtinger, Annette
Janssen, Klaus-Peter
Kuppen, Peter JK
van de Velde, Cornelis JH
Weirich, Gregor
Erlmeier, Franziska
Langer, Rupert
Aubele, Michaela
Zitzelsberger, Horst
Aichler, Michaela
Walch, Axel
Titel
High-resolution MALDI-FT-ICR MS imaging for the analysis of metabolites from formalin-fixed, paraffin-embedded clinical tissue samples
Ist Teil von
The Journal of pathology, 2015-09, Vol.237 (1), p.123-132
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2015
Link zum Volltext
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
We present the first analytical approach to demonstrate the in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded (FFPE) human tissue samples. Using high‐resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry imaging (MALDI‐FT‐ICR MSI), we conducted a proof‐of‐principle experiment comparing metabolite measurements from FFPE and fresh frozen tissue sections, and found an overlap of 72% amongst 1700 m/z species. In particular, we observed conservation of biomedically relevant information at the metabolite level in FFPE tissues. In biomedical applications, we analysed tissues from 350 different cancer patients and were able to discriminate between normal and tumour tissues, and different tumours from the same organ, and found an independent prognostic factor for patient survival. This study demonstrates the ability to measure metabolites in FFPE tissues using MALDI‐FT‐ICR MSI, which can then be assigned to histology and clinical parameters. Our approach is a major technical, histochemical, and clinicopathological advance that highlights the potential for investigating diseases in archived FFPE tissues. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Sprache
Englisch
Identifikatoren
ISSN: 0022-3417
eISSN: 1096-9896
DOI: 10.1002/path.4560
Titel-ID: cdi_proquest_miscellaneous_1702651883
Format
–
Schlagworte
Biomarkers, Tumor - metabolism
,
cancer
,
Cluster Analysis
,
Computational Biology
,
Cyclotrons
,
Diagnosis, Differential
,
Disease-Free Survival
,
Female
,
Fixatives - chemistry
,
Formaldehyde - chemistry
,
formalin-fixed paraffin-embedded tissue
,
Fourier Analysis
,
Germany
,
Humans
,
imaging mass spectrometry
,
MALDI
,
Male
,
metabolite
,
Metabolomics - methods
,
Neoplasms - metabolism
,
Neoplasms - mortality
,
Neoplasms - pathology
,
Neoplasms - therapy
,
Netherlands
,
Paraffin Embedding
,
Predictive Value of Tests
,
Proportional Hazards Models
,
Reproducibility of Results
,
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
,
Time Factors
,
Tissue Fixation - methods
,
Treatment Outcome
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