Details

Autor(en) / Beteiligte

• Peters, Ruud J. R. W.
• Nijemeisland, Marlies
• van Hest, Jan C. M.

Titel

Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles

Ist Teil von

• Angewandte Chemie (International ed.), 2015-08, Vol.54 (33), p.9614-9617

Auflage

International ed. in English

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• External small‐molecule triggers were used to reversibly control dynamic protein–ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small‐molecule substrates, thereby modulating the pH‐sensitive interaction between a Ni‐NTA ligand on the vesicle membrane and an oligohistidine‐tagged protein in the lumen. By alternating the small‐molecule substrates the interaction could be reversed. Small‐molecule triggers are used to reversibly control protein–ligand interactions in giant vesicles (see picture; yellow and blue pentagons denote NADH and NAD+, respectively). The results show that GUVs can mimic the ability of cells to react to external stimuli by starting a signaling event to induce a reversible structural change inside the vesicle.