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Methylation Patterns of Immunoglobulin Genes in Lymphoid Cells: Correlation of Expression and Differentiation with Undermethylation
Ist Teil von
Proceedings of the National Academy of Sciences - PNAS, 1983-11, Vol.80 (21), p.6642-6646
Ort / Verlag
United States: National Academy of Sciences of the United States of America
Erscheinungsjahr
1983
Link zum Volltext
Quelle
EZB-FREE-00999 freely available EZB journals
Beschreibungen/Notizen
Different states of eukaryotic gene expression are often correlated with different levels of methylation of DNA sequences containing structural genes and their flanking regions. To assess the potential role of DNA methylation in the expression of immunoglobulin genes, which require complex rearrangements prior to expression, methylation patterns were examined in cell lines representing different stages of lymphocyte maturation. Methylation of the second cytosine in the sequence 5′C-C-G-G 3′was determined by using Hpa II/Msp I endonuclease digestion. Four CHgenes (Cμ, Cδ, Cγ2b, and Cα), Cκ, Vκ, Cλ, and Vλgenes were analyzed. The results lead to the following conclusions: (i) transcribed immunoglobulin genes are undermethylated; (ii) the C gene allelic to an expressed C gene is always also undermethylated; and (iii) all immunoglobulin loci tend to become increasingly undermethylated as B cells mature.