Plant physiology (Bethesda), 2013-01, Vol.161 (1), p.72-80
2013
Details
- Autor(en) / Beteiligte
- Titel
- Molecular Characterization of the Fatty Alcohol Oxidation Pathway for Wax-Ester Mobilization in Germinated Jojoba Seeds
- Ist Teil von
- Plant physiology (Bethesda), 2013-01, Vol.161 (1), p.72-80
- Ort / Verlag
- Rockville, MD: American Society of Plant Biologists
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- Jojoba (Simmondsia chinensis) is the only plant species known to use liquid wax esters (WEs) as a primary seed storage reserve. Upon germination, WE hydrolysis releases very-long-chain fatty alcohols, which must be oxidized to fatty adds by the sequential action of a fatty alcohol oxidase (FAO) and a fatty aldehyde dehydrogenase (FADH) before they can be β-oxidized. Here, we describe the cloning and characterization of genes for each of these two activities. Jojoba FAO and FADH are 52% and 68% identical to Arabidopsis (Arabidopsis thaliana ) FAO3 and ALDH3H1, respectively. The genes are expressed most strongly in the cotyledons of jojoba seedlings following germination, but transcripts can also be detected in vegetative tissues. Proteomic analysis indicated that the FAO and FADH proteins can be detected on wax bodies, but they localized to the endoplasmic reticulum when they were expressed as amino-terminal green fluorescent protein fusions in tobacco (Nicotiana tabacum) leaves. Recombinant jojoba FAO and FADH proteins are active on very-long-chain fatty alcohol and fatty aldehyde substrates, respectively, and have biochemical properties consistent with those previously reported in jojoba cotyledons. Coexpression of jojoba FAO and FADH in Arabidopsis enhanced the in vivo rate of fatty alcohol oxidation more than 4-fold. Taken together, our data suggest that jojoba FAO and FADH constitute the very-long-chain fatty alcohol oxidation pathway that is likely to be necessary for efficient WE mobilization following seed germination.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889, 1532-2548eISSN: 1532-2548DOI: 10.1104/pp.112.208264Titel-ID: cdi_proquest_miscellaneous_1273304240
- Format
- –
- Schlagworte
- Alcohol Oxidoreductases - genetics, Alcohol Oxidoreductases - metabolism, Aldehyde Oxidoreductases - metabolism, Aldehydes, Arabidopsis - enzymology, Arabidopsis - genetics, BIOCHEMISTRY AND METABOLISM, Biological and medical sciences, Cloning, Molecular, Cotyledon - genetics, Cotyledon - metabolism, Cotyledons, Endoplasmic Reticulum - metabolism, Enzyme Activation, Enzymes, Epidermal cells, Esters, Esters - metabolism, Fatty acids, Fatty alcohols, Fatty Alcohols - metabolism, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant, Genetic aspects, Germination, Jojoba, Magnoliopsida - enzymology, Magnoliopsida - genetics, Nicotiana - enzymology, Nicotiana - genetics, Oxidation, Oxidation-Reduction, Plant Leaves - enzymology, Plant Leaves - genetics, Plant physiology, Plant physiology and development, Plants, Genetically Modified - enzymology, Plants, Genetically Modified - genetics, Properties, Proteins, Proteomics - methods, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, Seedlings, Seeds, Seeds - genetics, Seeds - metabolism, Waxes, Waxes - metabolism