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Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model
Ist Teil von
Probiotics and antimicrobial proteins, 2021-12, Vol.13 (6), p.1644-1657
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2021
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Probiotics, in particular
Lactobacillus
(lactic acid bacteria, LAB) strains, are widely used in clinical practice. Despite that these probiotics have GRAS (generally regarded as safe) and qualified presumption of safety (QPS) statuses, the safety of particular strains still needs to be thoroughly studied. The aim of the study was to evaluate the safety of
Lact. casei
IMV B-7280 strain by investigating toxicity and the effects on gut microbiota in experimental animal model. Male BALB/c mice (7–8 weeks, weight 20–24 g) were treated with amounts of
Lact. casei
IMV B-7280 strain: 5 × 10
6
, 5 × 10
8
, or 5 × 10
9
CFU/animal once per day during 7 days, or in the amount of 1 × 10
10
CFU/animal once per day during 3 days (most of the proposed probiotic doses for humans—from 10
8
to 10
9
CFU) and monitored during 14 days. Blood tests and serum biochemistry were conducted; the cecal content from mice of the experimental and control groups were freshly collected and analyzed. At the end of the experiments (15th day), the presence of LAB in the heart, liver, kidney, and mesenteric lymph nodes and peripheral blood was determined; histology of the brain, liver, heart, fragments of the small and large intestine, and mesenteric lymph nodes was conducted. Survival rate of BALB/c mice treated with
Lact. casei
IMV B-7280 strain in different concentrations in toxicity experiments during 14 days was 100%. We observed no signs of toxicity as changes in gait, lethargy, sleep, somatomotor activity as well as changes in fur, eyes, skin and mucous membranes, tremors, behavior pattern, convulsions, salivation, diarrhea, and local injuries in mice from all experimental groups. After administration of probiotic strain, the number of opportunistic bacteria in cecal contents, such as
Staphylococcus
spp.,
Candida
spp.,
Pseudomonas
spp., and total aerobic and optionally anaerobic bacteria decreased compared to controls; the population of beneficial bacteria such as lactobacilli increased in cecal contents of these mice. LAB were not detected in the peripheral blood, heart, liver, kidneys, and mesenteric lymph nodes after administration of this strain to intact mice.
Lact. casei
IMV B-7280 strain is safe at dose up to 10
10
CFU/animal during 3- and 7-day oral administration to mice and has a positive effect on the gut microbiota composition; it could be potentially considered as safe probiotic for humans.