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Determination of Gallic Acid in Rotala rotundifolia and Shuijia Zhixue Capsule by HPLC
Ist Teil von
Medicinal plant, 2019-08, Vol.10 (4), p.60-65
Ort / Verlag
Cranston: Wu Chu (USA-China) Science and Culture Media Corporation
Erscheinungsjahr
2019
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
According to the literature in recent ten years, high performance liquid chromatography (HPLC) is the main method for the determination of gallic acid&8 15]. HPLC method has the advantages of strong selectivity, high detection sensitivity, good separation effect and so on. [...]this paper established a method for the determination of gallic acid in R. rotundifolia and Shuijia Zhixue capsule, which laid the foundation for the follow-up analysis of R. rotundifolia and Shuijia Zhixue capsule. 2Materials and methods 2.1 Materials 2.1.1 Instruments. According to the detection results, the relative standard deviation (RSD) of gallic acid concentration was 1. In the existing literature, the content of gallic acid is mostly determined by HPLC method using acetonitrile and methanol as organic phase, water, phosphoric acid solution and glacial acetic acid solution as aqueous phase. [...]we took the chromatographic peak separation of the sample as an index to screen the mobile phase and its proportion.