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Exposure to perflubron is associated with decreased Syk phosphorylation in human neutrophils
Ist Teil von
Journal of applied physiology (1985), 2001-11, Vol.91 (5), p.1941-1947
Ort / Verlag
Bethesda, MD: Am Physiological Soc
Erscheinungsjahr
2001
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
Departments of 1 Emergency Medicine, 2 Pathology, and
3 Surgery, University of Michigan, Ann Arbor, Michigan
48109-0303
Liquid ventilation with perflubron
is associated with reduced neutrophil recruitment into the lung during
acute injury. Perflubron also reduces chemotactic responses, the
respiratory burst, and cytokine production in neutrophils and in
alveolar macrophages in vitro. In the current studies, the effect of
perflubron on neutrophil chemotaxis to formyl-Met-Leu-Phe
(fMLP) and phagocytosis of opsonized sheep erythrocytes (EA)
correlated with decreased phosphorylation of Syk, an important
intracellular second messenger in pathways regulating neutrophil
functional responses. Brief (5 min) exposure of neutrophils to
perflubron resulted in a dose-dependent reduction in chemotaxis to fMLP
and reduced phagocytosis of EA but no apparent morphological changes as
seen by electron microscopy. Concurrently, there was a reduction in
both total cytosolic tyrosine phosphorylation and Syk phosphorylation.
Binding studies indicated that this effect was neither a result of
impaired ligand-receptor affinity nor a change in the number of fMLP
receptors available on the neutrophil surface. These results suggest
that perflubron nonspecifically affects cellular activation as measured
by tyrosine phosphorylation perhaps by interfering with transmembrane
signal transduction.
partial liquid ventilation; perfluorocarbon; acute lung injury; tyrosine kinase; Fc RIIA