Details

Autor(en) / Beteiligte

• Wang, Yuefeng
• Xie, Fengfeng
• Zhou, Yuqing
• Du, Peilin
• Zhu, Hua
• Teng, Jianbei

Titel

SSR Primer Screening for Marchantia convoluta by SAM Method

Ist Teil von

• Agricultural biotechnology (Pawtucket, R.I.), 2017-10, Vol.6 (5), p.1-5

Ort / Verlag

Cranston: Wu Chu (USA-China) Science and Culture Media Corporation

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [ M ethods] The M. convoluta genomic DNA w a extracted by improved CTAB method，a d the SSR primers for M. convoluta were selected through digestion，ligation, suppression PCR, pre-amplification, SAM method a d PCR. [R eu lts] The improved CTAB method extracted a high purity of DNA； t e SAM PCR method gave clear bands； and 100 positive clones were sequenced，obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for aalyzing the genetic diversity of M. convoluta.