Details
- Autor(en) / Beteiligte
- Titel
- Mitochondrial reactive oxygen species modulate mosquito susceptibility to Plasmodium infection
- Ist Teil von
- PloS one, 2012-07, Vol.7 (7), p.e41083
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Mitochondria perform multiple roles in cell biology, acting as the site of aerobic energy-transducing pathways and as an important source of reactive oxygen species (ROS) that modulate redox metabolism. We demonstrate that a novel member of the mitochondrial transporter protein family, Anopheles gambiae mitochondrial carrier 1 (AgMC1), is required to maintain mitochondrial membrane potential in mosquito midgut cells and modulates epithelial responses to Plasmodium infection. AgMC1 silencing reduces mitochondrial membrane potential, resulting in increased proton-leak and uncoupling of oxidative phosphorylation. These metabolic changes reduce midgut ROS generation and increase A. gambiae susceptibility to Plasmodium infection. We provide direct experimental evidence indicating that ROS derived from mitochondria can modulate mosquito epithelial responses to Plasmodium infection.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0041083Titel-ID: cdi_plos_journals_1326223641
- Format
- –
- Schlagworte
- Aedes - metabolism, Aedes - parasitology, Animals, Anopheles, Anopheles - metabolism, Anopheles - parasitology, Anopheles gambiae, Anti-Bacterial Agents - chemistry, Aquatic insects, Bacterial infections, Biology, Biosynthesis, Cells, Cells (Biology), Cloning, Molecular, Culicidae, Disease susceptibility, Enzymes, Gene expression, Gene Silencing, Health aspects, Hydrogen Peroxide - chemistry, Infection, Infections, Infectious diseases, Laboratories, Malaria, Malaria - metabolism, Medicine, Membrane potential, Metabolism, Midgut, Mitochondria, Mitochondria - metabolism, Mitochondrial Membrane Transport Proteins - genetics, Mitochondrial Membrane Transport Proteins - metabolism, Models, Biological, Molecular Conformation, Mosquitoes, Oxidation, Oxidation-Reduction, Oxidative Phosphorylation, Oxygen, Oxygen Consumption, Parasites, Phosphorylation, Phylogeny, Physiological aspects, Plasmodium, Plasmodium berghei - metabolism, Plasmodium cynomolgi, Protein transport, Proteins, Protons, Protozoan Proteins - genetics, Protozoan Proteins - metabolism, Reactive Oxygen Species, Respiration, RNA, Double-Stranded - metabolism, Saccharomyces cerevisiae, Transporter, Yeast