PloS one, 2009-11, Vol.4 (11), p.e7854-e7854
2009
Details
- Autor(en) / Beteiligte
- Titel
- Blood-Feeding Induces Reversible Functional Changes in Flight Muscle Mitochondria of Aedes aegypti Mosquito
- Ist Teil von
- PloS one, 2009-11, Vol.4 (11), p.e7854-e7854
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- Background: Hematophagy poses a challenge to blood-feeding organisms since products of blood digestion can exert cellular deleterious effects. Mitochondria perform multiple roles in cell biology acting as the site of aerobic energy-transducing pathways, and also an important source of reactive oxygen species (ROS), modulating redox metabolism. Therefore, regulation of mitochondrial function should be relevant for hematophagous arthropods. Here, we investigated the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and yellow fever. Methodology/Principal Findings: Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an event that was parallel to blood digestion. These changes were most intense at 24 h after blood meal (ABM), the peak of blood digestion, when oxygen consumption was inhibited by 68%. Cytochromes c and a+a3 levels and cytochrome c oxidase activity of the electron transport chain were all reduced at 24 h ABM. Ultrastructural and molecular analyses of FM revealed that mitochondria fuse upon blood meal, a condition related to reduced ROS generation. Consistently, BF induced a reversible decrease in mitochondrial H2O2 formation during blood digestion, reaching their lowest values at 24 h ABM where a reduction of 51% was observed. Conclusion: Blood-feeding triggers functional and structural changes in hematophagous insect mitochondria, which may represent an important adaptation to blood feeding.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0007854Titel-ID: cdi_plos_journals_1292877063
- Format
- –
- Schlagworte
- Aedes - metabolism, Aedes - physiology, Aedes aegypti, Animal Feed, Animal Nutrition Sciences, Animals, Aquatic insects, Arthropoda, Arthropods, Autophagy, Biochemistry, Biochemistry/Chemical Biology of the Cell, Biochemistry/Membrane Proteins and Energy Transduction, Biological effects, Blood, Blood - metabolism, Cell Biology/Cellular Death and Stress Responses, Culicidae, Cytochrome, Cytochrome-c oxidase, Cytochromes, Dengue, Digestion, Drosophila, Electron transport, Electron transport chain, Electron Transport Complex IV - metabolism, Feeding, Fever, Flight, Flight muscle, Flight, Animal, Gene expression, Hematophagy, Hydrogen peroxide, Hydrogen Peroxide - chemistry, Insects, Mammals, Metabolism, Microscopy, Electron, Transmission - methods, Mitochondria, Mitochondria, Muscle - metabolism, Models, Biological, Morphology, Mosquitoes, Oxidation-Reduction, Oxygen, Oxygen Consumption, Phosphorylation, Rabbits, Reactive Oxygen Species, Reduction, Reverse Transcriptase Polymerase Chain Reaction, RNA - metabolism, Structure-function relationships, Tropical diseases, Vector-borne diseases, Viral diseases, Yellow fever